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Hemodialysis set-up 55 Thermal Biosensors in Biotechnology urea [mmol/l] Fig. 13. Set-up of a Hemodialysis simulation clinical range: 1,7 mmol/l–8,3 mmol/l time [h] Fig. 14. On-line-data of a hemodialysis experiment 56 F. Lammers · Th. Scheper time measurements at hospitals. Moreover, patients individual urea level and ureases long term stability encourages one to employ the method in routine treatment. 4 Kinetic Characterization of Immobilized Biocatalysts Immobilized enzymes are not restricted to bioanalytical applications.

1 High Resolution Thin-Film Thermistors . . . . . . . . 2 Miniaturized Enzyme Thermistors . . . . . . . . . . 3 Integrated Thermopiles . . . . . . . . . . . . . 62 Advances in Biochemical Engineering / Biotechnology, Vol. 64 Managing Editor: Th. Scheper © Springer-Verlag Berlin Heidelberg 1999 36 F. Lammers · Th. 4 Bio-Thermochips . . . . . . . . . . . . . . . 5 Compact Multichannel Enzyme Thermistors . . . . . . . 63 6 Conclusions . . . . .

In the initial experiments, very simple devices were used. Partly, they had unfavourable response-times, a complicated thermostating, small sample frequencies and an irregular baseline. The thermistor was fixed at the tip of a flow through coil (cartridge or tube with immobilized enzyme). The enzymatic reaction takes places in the coil and is accompanied by heat production. The flowing medium transports the resulting temperature gradient to the fixed thermistor that detects the local temperature change.

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