By Ute Resch-Genger, Markus Grabolle, Roland Nitschke, Thomas Nann (auth.), Alexander P. Demchenko (eds.)

ISBN-10: 3642046991

ISBN-13: 9783642046995

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“This e-book is a complete evaluation of the different sorts of nanoscale probes which were and are at present being built for sensing of analytes. The well timed reference part permits the reader to find key references to the first literature for growth of the cloth lined. … a helpful source that covers the factitious, photo-physical, and theoretical elements of this various box of research.” (Jeffrey T. Petty, magazine of the yank Chemical Society, Vol. 133, 2011)

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Additional resources for Advanced Fluorescence Reporters in Chemistry and Biology II: Molecular Constructions, Polymers and Nanoparticles

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Wang Q, Xu Y, Zhao X, Chang Y, Liu Y, Jiang L, Sharma J, Seo DK, Yan H (2007) A facile one-step in situ functionalization of quantum dots with preserved photoluminescence for bioconjugation. J Am Chem Soc 129:6380–6381 39. Xing Y et al (2007) Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry. Nat Protoc 2:1152–1165 40. Nann T (2005) Phase-transfer of CdSe@ZnS quantum dots using amphiphilic hyperbranched polyethylenimine. Chem Commun:1735–1736 41. Nann T, Mulvaney P (2004) Single quantum dots in spherical silica particles.

Changes in temperature typically result only in small spectral shifts, yet in considerable changes in the fluorescence quantum yield and lifetime. This sensitivity can be favorably exploited for the design of fluorescent sensors and probes [24, 51], though it can unfortunately also hamper quantification from simple measurements of fluorescence intensity [116]. , circumvented by ratiometric measurements [24, 115]. The microenvironment dependence of the optical properties of organic fluorophores is controlled by dye class, nature of the emitting state(s), excited state redox potential, charge, and hydrophilicity.

Examples are enzyme-catalyzed labeling by posttranslational modification, as in biotin ligase-catalyzed introduction of biotin into biotin acceptor peptides, which may be used to label proteins at the cell surface. Both intracellular and surface labeling have also been achieved by specific chelation of membrane-permeant fluorescent ligands (biarsenical dyes such as FIAsH or ReAsH bind to the tetracysteine motif, Ni-nitriloacetic acid (NTA) conjugates bind to the hexahistidine motif, and Zn conjugates), or by self-labeling, in which proteins fused to O6-alkylguanine-DNA alkyltransferase are combined with enzymatic substrate derivatives (O6-alkylguanine-DNA alkyltransferase (AGT) or SNAP-tags) [1, 99].

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Advanced Fluorescence Reporters in Chemistry and Biology II: Molecular Constructions, Polymers and Nanoparticles by Ute Resch-Genger, Markus Grabolle, Roland Nitschke, Thomas Nann (auth.), Alexander P. Demchenko (eds.)


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